梨树腐烂病是危害梨树生产的重要病害之一，为探寻梨树腐烂病(Pear valsa canker)的绿色防治药剂，室内采用菌丝生长速率法和离体枝条打孔接种法，测定了不同浓度的黄连水浸提液对梨树腐烂病菌菌落生长的影响及其在离体梨树枝条上的保护作用。结果表明，不同浓度的黄连水浸提液对梨树腐烂病菌均有不同程度的抑制作用，其中浓度为5.000 0%时抑菌率高达100%；当黄连水浸提液浓度为5.000 0%和10.000 0%时，病疤面积间无显著差异，室内防效分别高达75.8%和78.2%。表明不同浓度的中药材黄连水浸提液对梨树腐烂病均有一定防效，浓度为5.000 0%时对菌丝的抑制作用和离体枝条保护作用最为经济有效。

Pear valsa canker is one of the major diseases in pear producton. In search of green control agents for pear valsa canker, the effects of different concentrations of water extract of C. chinensis on the colony growth of V. pyri and its protective effect on isolated pear branches were determined by using mycelial growth rate method and excised twigs inoculation in vitro. The results showed that the different concentrations of water extract of C. chinensis had different degrees of inhibition on V. pyri, among which the inhibition rate was up to 100% at the concentration of 5.000 0%. The results of the in vitro branch protection test showed that there was no significant difference between the area of disease scars when the concentration of water extract of C. chinensis was 5.000 0% and 10.000 0%, and the preventive effect on detached shoots with the efficiency was up to 75.8% and 78.2%, respectively. This study showed that different concentrations of water extract of Coptis chinensis had certain effect on pear valsa canker, and the inhibition of mycelium and protection of isolated branches at a concentration of 5.000 0% is the most economical and effective.

S436.612.1；S567.5

甘肃省自然科学基金(20JR10RA458)；甘肃省农业科学院创新专项(2020GAAS38、2021GAAS17)；国家重点研发计划(2018YFD0201404-4)。