半夏根尖染色体压片效果受多重因素制约，为方便半夏染色体倍性鉴别、染色体计数，探索适用于半夏的根尖压片技术选取半夏根尖为材料，采用植物染色体常规压片法，对预处理液、预处理时间、固定时间、染色液以及染色时间5个主要参数进行一系列的筛选优化。结果表明，在半夏根尖压片过程中，用0.002 mol/L 8-羟基喹啉溶液预处理3 h，卡诺氏固定液固定20.0 h，60 ℃水浴，1 mol/L盐酸酸解10 min，45%醋酸软化20 min，最后用改良石炭酸复红染液染色20 min，压片效果显著，染色体清晰可辨，可用于半夏染色体观察研究。

The effect of Pinellia root tip chromosome compression is restricted by multiple factors. In order to facilitate the identification of Pinellia chromosomal ploidy and chromosome counting, a root tip compression technology suitable for Pinellia ternate was explored. In this experiment, the root tips of Pinellia ternate were selected as the material, and the routine pressing method of plant chromosomes was used. A set of technical methods for Pinellia root apex compression was developed. The experimental results showed that: in the process of root tip compression of Pinellia ternate, when pretreated with 0.002 mol/L 8-hydroxyquinoline solution for 3 h, fixed with Carnot's fixative for 20.0 h, water bath at 60 ℃, and acid hydrolyzed in 1 mol/L HCl for 10 min, softened in 45% acetic acid for 20 min, and finally stained with modified carbolic acid fuchsin for 20 min, the tableting effect was remarkable, and the chromosomes were clearly distinguishable, which can be applied to the observation and research of Pinellia ternate.

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国家中药材产业技术体系建设专项资金资助(CARS-21)；2021年甘肃省第五批重点研发计划项目(21YF5NA130)。