为了探究兰州百合试管鳞茎膨大培养基最佳配方和培养条件，解决试管苗移栽成活率低的问题，以兰州百合鳞片诱导形成的小鳞茎为试验材料，研究光暗不同培养条件下蔗糖和大量元素对兰州百合试管鳞茎生长和膨大的影响。在光照(12 h/d光照)和黑暗(24 h/d黑暗)条件下，设置不同质量浓度蔗糖(90 g/L、120 g/L、150 g/L)和不同浓度的大量元素(MS、2MS、3MS)组合处理，培养60 d后测定其鳞茎直径、鲜重、叶长等相关指标。结果表明，蔗糖和大量元素均对兰州百合试管鳞茎生长和膨大有显著的影响，且两者存在显著的交互作用，光照条件下鳞茎生长指数和鳞茎鲜重百分比均大于黑暗条件。根据生产实际，推荐适合兰州百合鳞茎膨大的组合为2MS+蔗糖120 g/L+NAA 0.2 mg/L，培养条件为1 500～2 000 lx下光照12 h/d。

In order to explore the optimal medium formula and conditions for Lanzhou Lily (Lilium davidii var. unicolor) bulb enlargement in vitro, and to address theissue of low survival rate of tube seedling transplantation, the effects of sucrose and macro elements on bulb growth and enlargement of Lanzhou Lily in vitro were researched under different cultural conditions and the bulblets of Lanzhou Lily induced by scales were selected as experimental materials. The experiments were conducted using three different concentrations of sucrose(90 g/L, 120 g/L, 150 g/L) and different concentrations of macro element(MS, 2MS, 3MS) under the conditions of light (12 h/d light) and dark(24 h/d dark) and morphological indexes such as diameter, fresh weight, length of bulblets were measured after 60 d of culture. The results showed that sucrose and macro elements had significant effects on the growth and enlargement of in vitro bulblets, furthermore, there was a significant interaction between them. The growth index and percentage of bulb fresh weight under light condition were higher than those under dark condition. According to the actual production situation, the suitable combination for bulb enlargement of Lanzhou Lily was 2MS plus 120 g/L sucrose plus 0.2 mg/L NAA, and the illumination intensity was 1 500 to 2 000 lx for 12 h/d.

S644.1

陇原青年创新创业人才项目(2022LQGR16)；甘肃省重点研发计划项目(22YF7NA028)。