为筛选制定出亚麻叶中五种活性物质的最佳提取工艺，以亚麻叶中提取的荭草素、绿原酸、异荭草素、牡荆素、异牡荆素5种成分的含量作为指标，通过单因素试验研究含醇量、提取时间、固液比、提取次数、提取温度对5种成分提取效果的影响；并利用L9(34)正交试验法方法，优选最佳的提取工艺。确定的最佳提取工艺为乙醇溶液浓度75%、料液比1 ∶ 15(g/mL)、提取次数2次、提取时间1.5 h、温度80 ℃。该提取工艺是一种适合于亚麻叶中有效成分提取的有效方法，可为亚麻叶研究和开发利用提供参考。

To optimize the extraction process of five active substances from flax leaves, taking the contents of orientin, chlorogenic acid, isoorientin, vitexin and isovitexin extracted from flax leaves as indicators, the effects of alcohol content, extraction time, solid-liquid ratio, extraction times, and extraction temperature on the extraction effects for the five substances were studied by single factor experiment, the optimum extraction process was selected by L9 (34) orthogonal method. Results showed that the optimum extraction process was determined as ethanol content at 75%, solid-liquid ratio at 1∶15 (g/mL), extraction for 2 times and extraction time for 1.5 h at the temperature of 80 ℃. This extraction process is an effective method suitable for the extraction of effective substances from flax leaves, which can provide reference for the research, development and utilization of flax leaves.

S563.2

甘肃省天水市科技支撑计划(2020-NCK-7969)。