Lead pollution is one of the difficult problems to be solved urgently in soil heavy metal pollution at present. It is an effective method to repair lead-contaminated soil by interaction between microorganisms and trees. In this paper, the lead-tolerant endophytic bacteria from the stems and leaves of Phyllostachys pubescens were isolated and purified, and the strains were identified by physiological analysis and molecular identification. Results showed that a total of 27 endophytic bacteria were isolated under different concentrations of lead stress, which were positive by Gram staining and VP test, 24 strains were positive (+) and 3 strains were negative (-) in catalase test. The results of methylene red test showed that 4 strains were positive (++), 20 strains were weak positive (+) and 3 strains were negative(-), and the 16S rDNA sequence was amplified by PCR. By means of molecular identification methods such as BLAST, comparative analysis and establishment of phylogenetic tree with MEGA software, it was preliminarily inferred that the endophytic bacteria isolated were Pseudomonas, Bacillus cereus and Acinetobacter.