作为Anoctamin蛋白家族的第五个成员，ANO5基因主要在绵羊肌肉再生、肌母细胞融合和肌细胞膜修复中发挥作用。为探究ANO5基因在绵羊体内的功能，本研究利用生物信息学软件分析了绵羊ANO5基因及其编码产物的结构与功能。结果显示:绵羊ANO5基因含有一个最大长度为3 351 bp的开放阅读框，推测其编码1116个氨基酸残基。该基因编码蛋白的分子式为C5897H9014N1546O1668S42，分子质量为129 602.32 Da，理论等电点为7.24，估计半衰期为30 h，不稳定指数为49.71，脂肪族氨基酸指数为79.43。绵羊ANO5蛋白位于质膜的可能性最大(56.5%)，不含信号肽，具有8段跨膜区，属于不稳定亲水性跨膜蛋白。二级结构以无规卷曲(68.43%)为主，三级结构主要由无规卷曲缠绕折叠形成。

As the fifth member of the protein family Anostatin, the ANO5 gene mainly plays a role in sheep muscle regeneration, myocyte fusion, and muscle cell membrane repair. To explore the function of ANO5 gene in sheep, bioinformatics software was used in this study to analyze the structure and function of the ANO5 gene and its encoding product. The findings indicatesthat the ANO5 gene encodes 1116 amino acid residues and has an open reading frame with a maximal length of 3 351 bp. The protein that is encoded by this gene is C5897H9014N1546O1668S42 in molecular formula, 129 602.32 Da in molecular mass, 7.24 in theoretical isoelectric point, 30 hours in estimated half-life, 49.71 in instability index, and 79.43 in aliphatic amino acid index. The sheep ANO5 protein, which contains eight transmembrane domains, is an unstable hydrophilic transmembrane proteinand is most likely to be found in the plasma membrane (56.5%). It also lacks signal peptides. Random curling accounted for 68.43% of the secondary structurewhile winding folding, and random curling constituted the majority of the tertiary structure.

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甘肃农业大学大学生创新创业训练计划项目(202304027)。