为筛选出大豆萌发期抗旱性鉴定的适宜试验条件，以大豆品种陇中黄601、恳鉴豆15、九农29、长农16为供试材料，采用蛭石法和滤纸法进行发芽试验，运用内源转录间隔区(Internally Transcribed Spacer，ITS)序列分析法进行发芽床筛选，测定各供试大豆品种的发芽率、硬实豆数、发霉豆数，并进行了蛭石湿度和PEG-6000浓度筛选。结果表明，蛭石法发芽试验能明显减少大豆发芽试验中产生霉菌的种类与数量，适宜在大豆萌发期抗旱性鉴定中应用。且在蛭石湿度为170%、PEG-6000浓度为22%的条件下，不同大豆品种间的发芽率差异比较明显，硬实豆数和发霉豆数少，试验数据可靠，可作为大豆萌发期抗旱性鉴定试验的适宜条件。且在此条件下，4个供试大豆品种的抗旱性表现由强到弱依次为陇中黄601、九农29、长农16、恳鉴豆15。

To select appropriate experimental conditions for the identification of drought resistance during the germination period of soybeans, varieties Longzhonghuang 601, Kenjiandou 15, Jiunong 29, and Changnong 16 were used as test materials. Germination tests were conducted using vermiculite and filter paper methods, with screening of germination beds through Internally Transcribed Spacer (ITS) sequence analysis. The germination rate, number of hard beans, and number of moldy beans were measured, along with selection for vermiculite moisture and PEG-6000 concentration. Results showed that the vermiculite method significantly reduced the types and quantities of molds in soybean germination tests, making it suitable for drought resistance identification during the germination period. At a vermiculite moisture of 170% and PEG-6000 concentration of 22%, significant differences in germination rates among the soybean varieties were observed, with fewer hard and moldy beans, providing reliable test data. These conditions are suitable for drought resistance identification tests during the soybean germination period. Under these conditions, the drought resistance of the 4 soybean varieties ranked from strongest to weakest as followed Longzhonghuang 601, Jiunong 29, Changnong 16 and Kenjiandou 15.

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兰州市人才创新创业项目(2022-RC-64)；甘肃省农业科学院科研条件建设及成果转化项目(重点研发计划)(2021GAAS04)；2022年省级重点人才项目(农作物种质资源保护利用与人才培养)(2022RCXM083)；甘肃省农业科学院青年基金项目(2023GAAS34)。