为了筛选和优化香菇原种及栽培种的培养基质配方，采用D-optimal设计方法，以麦粒和木屑不同配比为原料优化香菇原种培养基质，以木屑、玉米芯、麸皮不同配比为原料优化香菇栽培种培养基质，以香菇品种L808作为供试菌种，分别以其菌丝萌发期、菌丝长速、满袋期为评价指标，通过对各评价指标的测量，建立了各配比基质与香菇培养基质配方响应值之间的回归模型，从而科学的优化出香菇原种及栽培种栽培基质的配方。试验结果表明，香菇原种栽培基质最优配方为50%麦粒+50%木屑；香菇栽培种栽培基质最优配方为37.69%玉米芯+23.33%麸皮+38.98%木屑。在以上2个配方的栽培基质接种后，香菇菌丝的生长旺盛，萌发期短、满袋期短，且理化性质较优，说明优化得到的栽培基质配方具有较高的可行性，该设计方法也在优化培养料配比上是科学并且可行的。

In order to screen and optimize the culture medium formula of Lentinula edodes original and cultivated species, the D-optimal design method was used to optimize the original culture medium of Lentinula edodes with different ratios of wheat grain and sawdust as raw materials, and the cultivation medium of Lentinula edodes with different ratios of sawdust, corncob and bran as raw materials. The Lentinula edodes variety L808 was used as the test strain, and the mycelial germination period, mycelial growth rate and full bag period were used as the evaluation indexes, respectively. Through the measurement of the indexes, the regression model between the ratio matrix and the response value of the Lentinula edodes culture medium formula was established, so as to scientifically optimize the formula of the original and cultivated species of Lentinula edodes. The results showed that the optimal formula of Lentinula edodes original culture was: 50% wheat grain + 50% sawdust; the optimal formula of Lentinula edodes cultivation was: 37.69% corncob+23.33% bran+38.98% sawdust. After inoculating the cultivation substrates of the above two formulas, the mycelium of Lentinula edodes grew vigorously, with a short germination period and a short full bag period, and the physicochemical properties were also superior, indicating that the optimized cultivation substrate formula is highly feasible. This design method is scientific and feasible in optimizing the ratio of cultivation materials.

S646.1

甘肃省科技计划项目(22CX8NA031)；国家重点研发计划课题(2021YFD1100503)；国家食用菌产业技术体系(CARS-20)。