解决桃重茬问题对老桃园的更新改造具有重大意义，通过添加不同浓度的改良QL营养液、添加不同浓度 IBA和利用不同基质配方对抗重茬优良砧木品种GF677进行无糖组培生根的影响试验，同时进行了传统组培生根和无糖组培生根的对比试验。结果表明，GF677无糖组培使用浓度为50.00 %改良QL营养液时生根效果最佳，生根率高达96.67 %，生根数为3.6根，根长为3.45 cm；当在营养液中添加的IBA浓度为1.25 mg/L时生根效果最佳，生根率高达96.67 %；生根数为4.13根，根长为3.60 cm；基质配方使用纯蛭石时生根效果最佳，生根率高达93.33 %，生根数为3.80根，根长为3.50 cm。同时还可以看出，无糖组培的生根率较传统组培大体均有所增加，其中以IBA浓度为1.25 mg/L时无糖组培的生根率最高，较传统组培差异达显著水平。桃砧木GF677无糖组培的移栽成活率为92.6%，较传统组培增加4.6个百分点。由此可见，合理利用无糖组培生根的关键因子(营养液、IBA浓度、基质配方)能使桃砧木GF677生根率及成活率明显得到提高，应大力推广应用。

Addressing the issue of peach heavy stubble is of great significance for the renewal and transformation of old peach orchards. Peach rootstocks are the basis for peach tree grafting. To provide a scientific basis for the rapid propagation of replantation-resistant peach rootstocks using sugar-free tissue culture technology, experiments were conducted on effects of adding different concentrations of improved QL nutrient solution, various concentrations of IBA, and different substrate formulations on the rooting of the superior GF677 rootstock. A comparative experiment between traditional tissue culture rooting and sugar-free tissue culture rooting was also carried out. Results showed that using 50.00% improved QL nutrient solution in sugar-free tissue culture resulted in the best rooting effect, with a rooting rate of 96.67%, an average of 3.6 in root number, and a root length of 3.45 cm. When the IBA concentration in the nutrient solution was 1.25 mg/L, the rooting effect was optimal, with a rooting rate of 96.67%, 4.13 in root number, and a root length of 3.60 cm. The best rooting effect was observed when pure vermiculite was used as the substrate, with a rooting rate of 93.33%, 3.80 in root number, and a root length of 3.50 cm. Additionally, it was found that the rooting rate in sugar-free tissue culture was generally higher than that in the traditional tissue culture, with the highest rooting rate occurring at 1.25 mg/L IBA, which showed a significant difference compared to traditional tissue culture. The transplant survival rate of GF677 peach rootstock in sugar-free tissue culture was 92.6%, which was an increase of 4.6 percentage points over the traditional tissue culture. Therefore, the rational use of key factors for sugar-free tissue culture rooting(nutrient solution, IBA concentration, and substrate formulation) can significantly improve the rooting rate and survival rate of GF677 peach rootstock, and should be widely promoted and applied.

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甘肃省农业科学院中青年基金项目(2022GAAS51); 国家桃产业技术体系(CARS-30)。